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A method has been developed for improved time resolution in
on-line microdialysis. Conventional microdialysis sampling once
every 10 or 20 minutes is not enough to describe a fast transmitter
release accurately. In the new method analysis of dopamine (DA) and
serotonin (5-HT) with 3 minute time resolution has been achieved.
The method is surprisingly simple: instead of using
one 10 - 20 uL
injection loop, a 14 port valve with 3 loops (between 1 - 4 uL)
has been used. Each loop
contains information of a unique time period.
Three parallel 50 x 1 mm
HPLC columns are used for fast analysis of neurotransmitters. Run
duration is shorter than filling time of the 3 loops. Handling data
and conversion to response plots is done by automated procedures.
No autosampler is required in an on-line setup.
- 221-011 -
Three min time resolution in on-line microdialysis
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ALEXYS On-line TR
System with 3 flow cells for
simultaneous
analysis when time resolution is an issue. Configuration has been applied in
ALEXYS on-line, TR.
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