ALEXYS Monoamines Analyser

Antec Leyden has developed integrated system solutions for the analysis of noradrenaline, dopamine, serotonin and metabolites. Method is suitable for analysis in microdialysates, or tissue homogenates after sample prep. Our analysers are optimised for small sample volumes and picomolar detection limits.

Parallel processing using a 2D LC-EC system

NA, DA, 5-HT and metabolites, one injection 2 chromatograms
2 channel system for highest selectivity and sensitivity

Fig. 1. One injection, two chromatograms. Analysis of mouse prefrontal cortex microdialysate using the ALEXYS Monoamines Analyser.

Table 1. Microdialysis System Solutions

Part no.	Description
180.0088A	ALEXYS Monoamines Analyser

Configuration

For the Monoamines analyser we selected the VT03 electrochemical cell. This cell not only has an excellent reproducibility but also the best possible detection sensitivity. We routinely inject 2-8 µL with a maximum sample use of less than 10 µL, therefore we selected a micro version of our AS 100 autosampler. We use a 1 (or 2) mm ID column at 50 (or 250) µL/min.

The ALEXYS system comprises the following parts:

AS 100 autosampler, cool, micro
LC 110 HPLC pump
OR 110 organiser rack
Clarity data acquisition software
DECADE II electrochemical detector, DCC
VT cell with GC WE, ISAAC

Depending on the configuration column switching valves are included where necessary.

Application notes

213-017 - Noradrenaline, dopamine, serotonin and metabolites
213-021 - Noradrenaline, dopamine and serotonin
221-004 - Dopamine and serotonin analysis

ALEXYS Monoamines Analyser

Parallel 2D analysis using different, optimised LC and ECD conditions in each dimension.

Read more how we inject 4 µL out of 5 µL sample.

System solutions from Antec Leyden .... naturally!