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Microdialysis of neurotransmitters in vivo has become an invaluable
tool to study neurotransmission in living brain. Cerebrospinal fluid
of conscious animals is sampled through a microdialysis device and
analysed by HPLC with electrochemical detection. Neurotransmitter
concentrations are often below the nanomolar and sometimes even
below picomolar concentration range. The sample volume is usually
limited by the dialysis flow rate (0.5 – 2 µL/min) and the temporal
resolution required. Available sample volume is typically between 1
– 15 µL.
Antec Leyden has developed system solutions for
the analysis of neurotransmitters and metabolites in
microdialysates. These systems are optimised for analysis of
small sample volumes and picomolar detection limits.
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