ALEXYS Carbohydrates Analyser

Antec Leyden has developed an integrated system solution for the analysis of mono, di- and simple polysaccharides. The system is dedicated to the analysis of these carbohydrates in food and beverages, but also suitable for analysis of functional groups in glycoproteins.

Carbohydrate Analyser

• Reproducible, carbonate free analysis
• Down to 10 nmole/L detection limit
• Flexcell with exchangeable gold electrode

Fig. 1. Overlay of 25 chromatograms of an artificial sweetener containing maltodextrin.

Table 1. Carbohydrate System Solutions

Configuration

For the analysis of carbohydrates we selected the Flexcell electrochemical cell. This cell has an exchangeable working electrode which is particularly useful in pulsed electrochemical detection. The electrode material tends to vanish over the years, in the process of pulsed amperometric detection. Exchanging an electrode disc only takes a few minutes. The HyREF is completely maintenance free even at pH 13! The ALEXYS Carbohydrates 1 (180.0052A) comprises the following parts:

• AS 110 autosampler, cool
• LC 110 HPLC pump
• OR 110 organiser
• Clarity data acquisition software
• DECADE II electrochemical detector
• Flexcell with Au WE, HyREF
• HPLC column with pre-column
• ALEXYS LC connection kit
• Polyethylene bottle kit

• 1 additional LC 110 pump for HPLC gradients
• OR 110 'dual channel' option (extra pulse damper)

Table 2. Columns for Carbohydrates

Application notes and posters

• 220-002 - The Analysis of Carbohydrates using anion-exchange chromatography and pulsed amperometric detection
• 221-007 - The Analysis of Carbohydrates using the ALEXYS LC-EC System

ALEXYS Carbohydrates 2

Binary gradient HPLC with polyethylene bottles and He degassing for displacement of CO₂.