HyREF
Maintenance-free reference electrode for VT-03 electrochemical flow cell

pH resistant (1 - 14)
compatible with 100% modifier
no trapping of air bubbles
easy 'plug and play' installation

A revolutionary new reference electrode has been developed for the VT-03 flow cell. This solid state reference electrode is integrated in the inlet block of the flow cell. Consequently, inclusion of bubbles in the flow cell is virtually impossible. The HyREF reference electrode is absolutely maintenance-free and fully compatible with extreme mobile phase conditions, such as high pH or modifier concentrations up to 100%.

Carbohydrate analysis
Its compatibility with high pH makes the HyREF particularly useful for carbohydrate analysis, at pH 12 - 14. While the Ag/AgCl reference electrode slowly dissolves at such high pH, the HyREF is stable and absolutely maintenance free under these extreme conditions. Carbohydrates can be analysed in the 'pulse mode' of the DECADE using a HyREF flow cell with a gold working electrode or by using the INTRO with a flow cell with copper working electrode. Conditions are given in Application Note 214-003 and 214-001.

Application at high modifier percentages
The HyREF is compatible with any modifier percentage, even up to 100%. The Ag/AgCl reference electrode with the saturated chloride salt bridge is not convenient for such extreme conditions. A typical example is the analysis of vitamins A, D, E and K using a HyREF flow cell with a glassy carbon working electrode. Conditions are given in Application Note 218-004 and 218-005.

Ordering information
Flow cells of the HyREF type are available with glassy carbon, platinum, gold, silver and copper working electrode. They can be used in the DECADE II and the INTRO electrochemical detector.